사시나무 우량클론 삽목묘의 생장관련 주요 대사물질 구명

Identification of Key Metabolites Involved in Superior Clonal Growth Performance of Rooted Cutting Seedlings of Populus davidiana

In order to identify major metabolic components involved in the regulation of superior clonal seedling’s growth of Populus davidiana, we compared various clonal growth parameters (total dry weight, stem dry weight, and leaf dry weight) with the levels of metabolites in either leaf or stem of the corresponding rooted cutting seedlings. In leaf tissues, the contents of sugars, including glucose, sucrose, and inositol were significantly correlated with stem dry weight. In contrast, the levels of amino acids in stems, glutamic acid, alanine, and threonine, were correlated with leaf dry weight. Leaf tissues of superior clones contained sugars at least 1.3-fold higher than regular clones, while stem tissues accumulated 1.2-fold higher levels of amino acids compared to regular clones. In addition, superior clones accumulated higher amount of metabolites in tissue-dependent manner; i.e. rythronic acid, malic acid, and petanedioic acid in leaves, and succinic and maleic acid in stems. In conclusion, our results showed that clonal growth performance of P. davidiana was correlated with different types of metabolites in tissue-dependent manner, suggesting that tissue-dependent metabolic profiling might be a prerequisite process for identifying metabolic markers for growth traits of P. davidiana.