브로콜리 화기 구조에 따른 소포자 밀도 및 발달 단계

Developmental Stage and Density of Microspore by Flower Structure in Broccoli Lines

Microspore-derived embryos (MDE) induction was investigated in 74 broccoli lines. The MDE induction was observed in only 18 broccoli lines. Especially, MDE was produced about 20 per petri dish in 08-8-8 and 08-8-33 lines. The influence of MDE induction was investigated by flower structure and bud size (S, 0.1~0.2; M, 0.2~0.4; L, 0.4~0.6 mm) in 08-8-8 (high MDE induction line) and 08-8-10 (no MDE induction line) lines. There was no difference in structure of flower bud between 08-8-8 and 08-8-10 lines, but M size flower bud has much longer stigma than petal in only 08-8-8 line. There was no observation of microspore at S size flower bud of 08-8-8 and 08-8-10 lines. The No. of MDE was correlated with the flower bud size. Microspore density of anther was much higher in the 08-8-8 than in the 08-8-10 line. Most of microspores in anther were at late uninucleate stage in 08-8-8 (91.3%) and in 08-8-10 line (45.7%).