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Variation of Anthocyanin Content and Estimation of Anthocyanin Content from Colorimeter among Strawberry Accessions

This study was conducted to find out an efficient and non-destructive method to select strawberry plants with high anthocyanin content in breeding programs. The total anthocyanin content in both fruit skin and whole fruit of fully ripe fruits was analyzed by HPLC in 19 strawberry cultivars, which were selected among germplasm as potential breeding materials based on their good horticultural traits. The anthocyanin content varied greatly among cultivars, ranging from 22.04 to 87.16 mg·100g-1 FW in fruit skin and from 1.98 to 30.16 mg·100g-1 FW in whole fruit. For the anthocyanin content of whole fruit, it was high in the order of ‘Sachinoka’, ‘Tamar’, ‘Amaou’ and ‘Summerberry’. A correlation coefficient of anthocyanin content between fruit skin and whole fruit was significantly high as 0.867 and, therefore, the anthocyanin content of whole fruit can be estimated by that of fruit skin. Besides, the anthocyanin content of whole fruit was strongly negatively correlated with L* (lightness, -0.791) and b* (yellowness, -0.772) values measured by portable colorimeter. Therefore, the portable colorimeter can be a rapid and nondestructive method to estimate anthocyanin content of strawberry fruits when selecting anthocyanin-enriched strawberry lines in strawberry breeding programs.