S-allele Specific PCR 분석에 의한 사과와 꽃사과 품종의 자가불화합성 유전자형 동정

Identification of Self-Incompatibility Genotypes in Apple and Crabapple Cultivars by S-allele Specific PCR Analysis

Apple (Malus ☓ domestica Borkh.) has gametophytic self-incompatibility (S) controlled by the multi-allelic S-locus. In the present study, S-genotypes of 24 apple cultivars including newly released Korean cultivars and seven crabapple cultivars were identified using S-allele specific polymerase chain reaction analysis. Twelve different S-alleles (S1, S2, S3, S5, S7, S9, S10, S16, S21, S23, S26, and S29) from 31 apple and crabapple cultivars were identified using 23 S-allele specific primers. Among them, S1 (41.7%), S3 (58.3%), S7 (29.2%), and S9 (54.2%) S-alleles were found to be common in 24 apple cultivars. The newly released Korean cultivars ‘Arisoo’ and ‘Hwangok’ were genetyped as S3S7 and S3S9, respectively. S-genotypes information obtained from the present study will be useful to select proper pollinzers for stable production of apple fruit and to design cross of breeding programs.