반수체 밀 육성을 위한 밀 및 옥수수 품종 반응

Variation in Genotypic Responses of Wheat and Maize for Wheat Haploid Production

Doubled haploid (DH) system is an effective tool in improving breeding efficiency and has been widely applied in wheat breeding programs. Wheat x maize hybridization is used for the production of wheat DH because of its efficiency and ease of application. We carried out an experiment to investigate genotype effect of wheat (Triticum aestivum) and maize (Zea mays) on efficiency of wheat haploid production. In various wheat x maize crosses, ten wheat and seven maize genotypes were tested. Haploid embryos were rescued and cultured for plant regeneration. Average seed set, embryo formation and haploid egeneration of five wheat varieties in crosses with a pollinator cv. Gangdaok were about 75%, 19% and 8.4%, respectively. Their haploid regeneration ranged from 3.7∼9.8% and cv. Jokyoung showed highest regeneration. Average seed set, embryo formation and haploid regeneration of seven maize genotypes in crosses with a parent cv. Jokyoung were about 78%, 18% and 9.6%, respectively. Their haploid regeneration ranged from 7.6∼12.9% and cv. Kwangpyeongok showed highest regeneration followed by cvs. Gangdaok and Gangilok. Analysis of variance for seed set and embryo formation showed highly significant effects of wheat parents and maize pollinators, whereas their interaction effect was only significant for seed set. The effect of maize genotypes on these traits was greater than that of wheat genotypes. Consequently, cvs. Kwangpyeongok, Gangdaok and Gangilok were found to be better pollen donors among the genotypes tested in wheat x maize hybridization for wheat hybrid production.