실리카 나노입자의 분산조건에 따른 입도분포 변화 및 A549 세포독성

Size Distribution of Silica Dioxide Nanoparticles after Calibrated Sonication and Cytotoxicity in Cultured A549 Cells

Size and/or size distribution is a very important factor in the cellular toxicity test of nanoparticles. Therefore, it is necessary to fulfill the requirement of standardized dispersion protocol when toxicity potencies of different types of nanoparticles are compared. In this study, different conditions including ethanol pre-wetting, concentration of bovine serum albumin in media, and measurement instruments were investigated to see the impact on the dispersity of silica nanoparticles after calibrated sonication. As results, the concentration of bovine serum albumin seemed to mostly influence the dispersion of silica nanoparticles. No significant difference was shown in cytotoxicity of cultured A549 cells among silica (SiO₂), zinc (ZnO), silver (Ag), ceria (CeO₂) and titanium (TiO₂) nanoparticles when they were dispersed by same amount of energy after calibrated sonication.