Fusarium oxysporum f. sp. raphani에 대한 무 유전자원의 저항성 평가

Evaluation of Resistance to Fusarium oxysporum f. sp. raphani in Radish Germplasm

Identification of Fusarium oxysporum f. sp. raphani and investigation on fusarium wilt development by isolates and inoculation methods were conducted to establish a screening method for fusarium wilt-resistance in radish (Raphanus sativus L.) germplasm. Pathogenicity of F. oxysporum f. sp. raphani isolate, Heonggye-1 and Heonggye-2, to radish plants was confirmed by seedling test. Radish seedlings were inoculated by root-dipping and soil-drenching with or without root-wounding. For Heonggye-1 isolate, mean disease indexes were 4.13 and 3.91 by root-dipping and soil-drenching with root-wounding, respectively, but those were 1.87 and 1.88 without root-wounding. For Heonggye-2 isolate, mean disease indexes were 3.83 to 4.37 regardless of inoculation methods. Two-hundred sixty accessions of radish germplasm collected from 9 countries of Asia and Europe were evaluated for fusarium wilt-resistance by soil-drenching with root-wounding with Heonggye-2 isolate. Fifty-four resistant accessions with higher than 70% of the percentage of resistant seedlings in accession (P R ) and lower than 20% of the percentage of susceptible seedlings in accession (P S ) was found. Eighteen susceptible accessions with lower than 20% of P R and higher than 50% of P S were selected. These accessions could be used as breeding and research materials after re-evaluation of disease-resistance and characterization of agronomical traits.