IMPROVED STABILITY AND IN VITRO SKIN PERMEATION OF ELASTIC LIPOSOMES LOADED WITH MAACKIA AMURENSIS EXTRACT

In this study, We prepared elastic liposome containing Maackia amurensis extract, Known as skin whitening effect, evaluated the vesicles size, elasticity, loading efficiency, stability, and in vitro skin permeation. Elastic liposome consisted of sodium-cholate and lyso-phospholipids (edge activator) was prepared in order to supplement stability defect of the conventional elastic liposome. In general, conventional elastic liposome are unstable. Lsophospholipids, sodium-cholate, cholesterol, diacylglycerols, and Tween80 were tested as edge activators. Of the edge activators, Sodium-cholate and lysophospholipids resulted more stable than did normally edge activators. The mean diameter of Maackia amurensis extract loaded elastic liposome formulation ranged between 150 ~ 250nm and loading efficiency was observed 65.5~81.8%. The hightest loading efficiency (81.8%) and deformability (25.6) were observed at the optimal ratio of 85 : 10 : 5(phospholipids : sodium-cholate : lyso-phospholipids) among 0.1% Maackia amurensis extract loaded elastic liposome formulation. Elastic liposome formulation was selected for further transdermal permeation study. Advance elastic liposome exhibited more skin permability than general liposome. These results suggest that elastic liposome formulation using sodium-cholate and lyso-phospholipids as a major edge activator could be useful for the delivery of active ingredient through the skin transdermal and improved stability of elastic liposome.