Solanum nigrum L.의 葯培養에 關한 硏究

Studies on the Anther Culture of Solanum nigrum L.

Solanum nigrum에서 半數體를 만들어 내기 위해 Murashige and Skoog의 改良 培地에 葯培養을 하였든 바 다음과 같은 결과를 얻었다. 小胞子는 培養 1 週後면 肥大하기 시작하고 계속 分裂해서 多細胞體로 되어 培養 약 2週日이면 白色光澤의 球形의 callus 葯을 헤치고 밖으로 나온다. 小胞子는 처음 2~4 細胞體 後에 多細胞體 등으로 변하지만 未分化 細胞의 集團이고 embryoid로서의 體制는 갖추지 못하고 callus로서 발달한다. embryoid로 발달하는가 또는 callus로 되는가는 첨가된 2,4-D등 生長調整物質의 농도와 관계가 있는 것 같다. 培地 위에 떨어진 callus의 일부는 활발하게 分裂하여 單細胞의 集團을 형성한다. 葯壁組織은 培養 中 細胞가 심하게 팽창되었을 뿐 callus化하는 것은 보이지 않는다. 植物體는 培養 約 6 週後 2,4-D를 除去한 培地에 移植한 callus에서 分化되기 始作했다.

The anthers of Solanum nigrum L. were cultured on the modified Murashige and Skoog’s medium, the results of which were summarized as follows: Some of the microspores examined one week after culture became large in size and had abundant cytoplasm. Later they turned into 2-, 4-, or multicelled bodies. In two weeks after culture white glowing ellipsoidal bodies emerged out of the anther and continued to grow as calli. It was not confirmed, however, that whether the spore grew into embryoid in its initial development and later changed to callus due to the effect of 2,4-D employed in the medium or the spore grew from the start into callus. Fresh calli in contact with the medium divided actively and gave rise to large mass of single cells on the surface of medium. 2n somatic callus originating from anther tissue was not observed in the materials examined several weeks after culture. Plantlet started to develop from the callus transferred to 2, 4-D free medium approximately six weeks after the initial inoculation of anther.