감마線을 照射한 小麥種子의 貯藏効果

Effects of Post-irradiation Storage on Wheat Seeds

小麥品種 Weibull’s Svenno 種子를 11.3%와 4.2%의 含水量으로 平衡시켜 감마線照射後 貯藏期間을 數分, 1日, 1週, 2週 및 4週로 하여 播種直前醱素 bubbling 하여 나타나는 貯藏效果를 苗生長에서 調査하였든바 얻어진 結果는 아래와 같다. 1) 放射線照射된 種子가 貯藏期問의 延長에 따라 生物學的障害는 크게 나타나며 이와 같은 傾向은 乾燥種子에서 더욱 뚜렷하게 보였다. 2) 始初의 生物學的障害程度는 種子水分含量에 無關하였으며 貯藏에 依하여 障害의 差異가 크게 나타났다. 이러한 結果는 突然變異育種硏究에 있어서 變更要因 卽 貯藏期問, 酸素效果 및 種子水分含量等에 대하여 考慮되어야함을 暗示하고 있다.

Storage effect in relation to moisture content was studied in gamma-irradiated wheat seeds. Variety used in the study is Weibull’s Svenno. Two seed moisture content was considered namely 4.2 percent and 11.3 percent. Prior to irradiation the seeds were equilibrated to this moisture content in a controlled humidity agent in a desiccator and irradiated at gamma doses ranging from 5 kR to 30 kR. After irradiation the following storage period were observed; within 10 minutes, 1 day, 1 week, 2 weeks and 4 weeks. After each indicated storage period the seeds were germinated in a growth chamber. Before sowing the seeds for germination, these were soaked in oxygenated water by bubbling oxygen at 20℃ for 5 hours. Seedling injury was observed to be vary with storage time. That is, long storage period the greater is the seedling injury. Seed moisture content was also observed to have similar effect as storage period. Least seedling growth was observed at low seed moisture content. It was observed that initial biological damage was not related to seed moisture content. Therefore, it was concluded that modifying factors such as storage period, oxygen, and moisture content of seed be considered in mutation breeding studies.