油菜Heterosis育種에 있어 母本繼代, 增殖을 爲한 組織培養 硏究 Ⅰ. 生長調節物質이 油菜莖租織片의 器官分化에 미치는 影響

Tissue Cultural studies on the Maintenance and Multiplication of Parental Lines in Rape (Brassica napus L.) Heterosis Breeding Ⅰ. Effect of Growth Regulators on the Organogenesis from Stem Segments of Rape by in Vitro

This study was conducted to explore the possibilities for the maintenance and multiplication of parental lines in rape(Brassica napus L.) heterosis breeding. Growth regulators influencing organogenesis were investigated in stem culture grown on a synthetic nutrient medium. Naphthaleneacetic acid(NAA), Indoleacetic acid (IAA) induced root, where as 2.4-Di chlorophenoxyacetic acid(2,4-D) didn’t induce. The whole plants were not induced when auxins such as NAA, IAA and 2.4-D, and cytokins such as benzyladenin(BA) and Kinetin were added individually. When NAA(0.5-2.0 ㎎/ℓ) was employed in conjunction with BA and Kinetin(1.0-2.5 ㎎/ℓ),whole plant was obtaind. The whole plant induction percentage were 56% and 11% in NAA 1.0 ㎎/ℓ+ BA 2.0 ㎎/ℓ and NAA 0.5㎎/ℓ+ Kinetin 2.0㎎/ℓ, respectively. Majority of whole plant were induced between 3-4 weeks after culture initiated.