器內培養에서 NAA와 BA가 땅콩의 器官分化 및 生長에 미치는 影響

Effects of NAA and BA on the Organogenesis and the Growth of Peanut (Arachis hypogaea) in In Vitro Culture

Naphthalenacetic acid ( NAA) and Benzyladenine (BA) have been known to have an important influence on the organogenesis and growth of plant, which has not been studied fully in peanut. This experiment was conducted to investigate the effects of N AA and BA on in vitro culture of peanut. In shoot tip culture, many leaves were expanded when treated with over 0.5 mg/1 BA, but the effect of BA on leaf expansion was inhibited with supplementing more than 0.5 mg/1 N AA. The best hormonal combination for leaf expansion was the 0.1mg/1 NAA+0.5mg/1 BA combination in cv. Yeongho, while 1mg/1 BA alone in cv. 01. Shoot elongation enhanced with addition of 0.1 mg/1 NAA or 0.1 mg/1 BA, but inhibited in the addition of more than 0.5 mg/1 BA. On the other hand, average internode length was reduced with the increasing BA concentration. In immature leaf culture, hormonal combination of 1 mg/1 N AA and 1/mg /1 BA was the most effective on callus and bud differentiation. Thirty days long culture was proper for bud differentiation. Optimum BA concentration was 1 mg /1 for shoot development from bud and proper culturing period was 40 days. Roots were hardly initiated from regenerated shoots and the effects of N AA on root initiation was insignificant.