벼 葯培養에어서 Callus 生育程度가 器管分化에 미치는 影響

Effects of Callus age on Plant Differentiation in Rice Anther Culture

Distribution of callus induction after anther inoculation and effect of callus age on plant differentiation were studied for improvment of culture efficiency in rice anther culture in vitro. N6 basic medium supplemented with 2㎎/ℓ of NAA and 1㎎/ℓ of kinetin used for callus induction, 0.1㎎/ℓ of IAA and 1㎎/ℓ of kinetin for plant differentiation. Varietal difference in callus induction was recognized, and the frequency of callus induction showed 86% from 20 days to 40 days after anther inoculation. This tendency also exists in F1 hybrids. The frequency of green plant differentiation was high at callus age with 11-20 days from callus induction to callus transplanting. Albino appeared at callus age with 21-40 days. The frequency of green plant differentiation showed 77% within 10 days after callus transplanting, but albino showed 71% within 20 days after callus transplanting.