학술저널
Colchicine을 利用한 水稻 葯培養 半數體의 染色體 倍加
Chromosome doubling of rice anther-derived haploid plants by colchicine treatment
- 한국육종학회
- 한국육종학회지
- Vol.17 No.1
-
1985.0367 - 69 (3 pages)
- 3
The addition of dimethyl sulfoxide(DMSO) and Tween 20 to colchicine solution applied to anther derived rice haploid plants increased the percentage of induced diploids more than did colchicine alone. When Gibberellic acid(GA 3) was added, plant mortality caused by Tween 20 was decreased, thus the percentage of haploids with doubled sectors was increased. Concentration of colchicine solution used was 0.1% and those of DMSO and Tween 20 were 2% and 0.03 %, respectively. At a concentration of 20 ㎎/ℓ, GA3 was more effective.
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