벼 葯培養에 있어서 低溫處理와 分化植物體의 倍數性

Effects of low temperature treatment on culturability and ploidy level in rice anther culture

This experiment was studied to determine the effects of low temperature treatment on culturability and ploidy level of anther-derived plants in rice anther culture. N₆ basic medium supplemented with 2mg/ℓ of NAA and 1mg/ℓ of Kinetin used for callus induction, 0.2mg/ℓ of IAA and 1mg/ℓ of Kinetin for plant differentiation. Rice panicle with flag leaf sheath was pretreated by low temperature at 10℃ for 15 days. Cold-shocked anthers produced more callus induction and plant differentiation about 3 fold than those without pretreatment. Callus induced from cold-shocked anthers produced more haploid plants and less diploid plants than the callus induced from anthers without pretreatment. This tendency was recognized in a variety as well as some F₁ crosses.