참깨 生長點 培養에 關한 硏究

Propagation of sesame(sesamum indicum L.) through shoot tip culture

This study was conducted to study the effect of NAA, IAA, 2,4-D, BA and kinetin on the callus induction and the organogenesis from the shoot tip of the sesame. In single treatment of NAA and IAA, NAA was better in shoot differentiation than IAA, but IAA was better in root differentiation. Among the various hormones used, kinetin 2.0 mg/ℓ was found to be the highest in shoot differentiation and NAA 0.5 mg/ℓ in whole plant induction percentage. The whole plant induction percentages were 86% and 29% in the combinations. of NAA 0.5 mg/ℓ + Kinetin 1.0 mg/ℓ and NAA 0.5 mg/ℓ + BA 1.0 mg/ℓ, respectively. Due to the interaction between NAA and kinetin, increase of NAA concentration reduced the shoot differentiation, but did not influence the root differentiation significantly. The most desirable medium was the MS medium containing 0.1 mg/ℓ NAA, 0.5 mg/ℓ IAA and 2.0 mg/ℓ kinetin. The whole plant induction percentage was 93% in this medium.