油菜와 芥子의 原形質體 融合에 關한 基礎硏究

Basic studies on somatic hybrids by protoplast fusion of brassica napus L. and brassica juncea L.

For the preliminary study on somatic hybrids by Brassica cell fusion as one of new breeding techniques, the conditions that would be most effective in isolation, fusion, and culture of Brassica napus and B. juncea protoplasts were examined. For the production of viable protoplasts from the leaf tissue, an enzyme mixture of 0.5% cellulase R-10 and 0.1% pectolyase Y-23, a 1 to 7 hour mannitol incubation period before enzyme tratment, and 0.5M mannitol as osmoticum in the cell enzyme tratment, and 0.5M mannitol as osmoticum in the cell enzyme mixture were optimum. A protoplasts fusion were obtained 30% PEG solution containing 0.4M glucose and 30mM CaCl₂. Cell divisions of the fusion protoplasts were continued and have generated colonies when they were grown S₁ medium.