학술저널
감자와 가지의 原形質體 融合
Protoplast fusion between patato and egg plants
- 한국육종학회
- 한국육종학회지
- Vol.18 No.4
-
1986.1210 - 13 (4 pages)
- 5
This study was carried out to find out the proper enzyme mix for protoplast isolation, and the effects of PEG, pH and Ca ion on the fusion. Mix of 2% cellulase R10 with 0.5% macerozyme R10 and 2.5% cellulase with 0.75% macerozyme were proper for isolation of protoplasts from mesophyll tissues of potato and egg plant, respectively. Fusion frequency was higher in each of 35% PEG(MW 6000), 15 mM Ca ion and at pH 5.5, however, their combined effect was not greater than as it was expected.
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