호박 1 代雜種의 純度檢定을 위한 phosphoglucomutase의 同位酵素 分析에 관한 硏究

The use of phosphoglucomutase isozymes analysis in the determination of the purity of F₁ seeds in sguash(cucurbita pepo L.)

Extracts from cotyledons of imbibied squash seeds of three commercial F₁ hybrids and their six parental inbred lines were analized by starch gel electrophoresis and stained for phosphoglucomutase(PGM;E.C-2.7.5.1) in order to investigate the possibility of use of the isozyme variants in determining purity of F₁ seeds. Diagnostic band variants for phosphoglucomutase were found in the most cathodal zone from the two parental inbreds of a F₁ hybrid. The band pattern and activity of PGM was stable during three days of germination. The band pattern of PGM was expressed by three loci. PGM-3 varients was controlled by two co-dominant alleles, PGM-3F and PGM-3S, at one locus.