벼 葯培養에 關한 硏究 Ⅰ. 花粉의 dimorphism과 葯培養

Studies on the anther culture of oryza sativa L. Ⅰ. Pollen dimorphism in anther culture

The influence of pollen dimorphism in rice anther culture was investigated from 15 crosses, japonica type F₁ generation, in winter of 1985. Pollen dimorphism could be observed plainly in size and staining reaction, and the number of the S pollen grains was the most at early stage 6, but the laggard and A type S pollen grains were partly developed into normal pollen grains just before flowering. The size of pollen grain was different by developmental stage and those of N and S pollen grains were 40 to 50μ and 30 to 40μ respectively just before flowering. The rate of callus formation was significantly increased according as the number of S pollen grains was grew up to 16%, and also the date of callus emergence from anther was faster, and it was especially related to B type S pollen. However the rate of callus formation in the anther donor with a lot of empty pollen grains was lower regardless of a number of S pollen grains. The correlation between S pollen grain rate and plant regeneration rate was not admitted.