Organ differentiation from callus of immature cotyledon in soybean, glycine max(L.) merr

The experiments were conducted to investigate organ differentiation from callus-derived embryoid under various media using immature cotyledon of soybean line Baemkong. 1.0mg 2,4-D plus 2.0 mg kinetin or 0.2mg BAP per litre on MS basal medium were effective growth regulators for callus induction. Meristematic structure was observed from the callus induced by 1.0mg 2,4-D plus 0.2mg kinetin per litre on MS basal medium in which callus growth showed best responses. Embryoid from yellowish green callus and leaflet from the embryoid were differentiated on the nutritional condition subcultured with 0.005 mg pieloram plus 2.0mg BAP per litre on MS basal medium after induction of callus oil the medium with 1.0 mg 2,4-D plus 2.0 mg kinetin per litre on MS in immature cotyledon.