煙草crown gall tumor 由來植物體의 特性

Characteristics of regenerants derived from crown gall tumor callus in nicotiana tabacum L.

Whole plants were regenerated from the crown gall tumor tissue induced from infection of tobacco by Agrobacterium tumefaciens in order to clarify the characteristics of tumor-originated plants. Petal of tumor plant was bigger than that of normal plants. And the transformed plants derived from the tumor tissue had more chlorophyll and carotenoid than normal plants. Superinfection of the tumor plant by the other strain of Agrobacterium tumefaciens C58 led to the crown gall tumor, which was round and smooth type. The shoots were directly formed from the explant of tumor plant on the redifferentiation medium. However, the callus derived from the explant of tumor plant did not formed the shoots on the same medium.