十字花科植物의 原形質體培養과 融合에 關한 硏究 Ⅱ. 山東菜와 갓의 原形質體培養에 의한 植物體 再生

Studies on protoplast culture and fusion in cruciferae Ⅱ. Plant regeneration from protoplast culture of brassica campestis and brassica juncea

The experiments were conducted to determine optimum concentration of various growth regulators in fresh and regeneration medium on which affect callus formation and plant regeneration from mesophyll protoplasts of Brassica campestris CV. “Koonweesandongchae” and Brassica juncea CV. “Kyungsangot”. The formation of microcallus from protoplast could be more successfully achieved when added weekly MS liquid medium containing 1.0mg/l NAA and 0.2mg/l BA in Brassica campestris and 0.2 mg/l 2.4-D, 1.0mg/l NAA and 0.2mg/l BA in Brassica juncea. Shoot differentiation from callus, 0.3cm in diameter was accomplished in MS medium containing 0.1mg/l IAA and 2.0mg/l Zeatin in Brassica campestris and 0.1mg/l IAA and 2.0mg/l Zeatin or 0.1mg/l NAA and 1.0mg/l BA in Brassica juncea.