煙草 crown gall tumor 由來 F₁植物體의 特性

Characteristics of F₁ progeny plants from tobacco tumor tissues

Plants were regenerated from callus tissues derived from tobacco crown gall tumor induced by Agrobacterium tumefaciens isolated from soil, Tumorous tobacco shoots were readily derived from tumor callus tissues. All of the regenerated shoots developed into mature flowering plants. Morphological feature of their flowers was similar to that of normal plants. When self-pollination was forced, the flowers degenerated into abortive fruits. A pollen analysis revealed that these flowers possessed a variable level of pollen sterility. When tumor plants were reciprocally cross-pollinated with normal plants, plants set seeds. In crosses of normal x tumor, normaltype F₁ progenies were obtained. However, crosses of tumor x normal gave F₁ progenies that showed retention of morphological of tumor plants.