油菜葯培養의 胚狀體誘起方法 改善

Improvement of embryoid inducing method in anther culture of brassica napus

Rape(Brassica napus) anthers were cultured for the improvement of embryoid induction. Modified B₅ medium was more effecient than modified MS medium for embryoid induction. In case of temperature treatment of anther prior to culture, high temperature at 35℃ for 2days was better than low temperature to improve embryoid induction. Media containing brassinolide or charcocal were not effective for embryoid induction of rape but medium supplemented with proline Ig/ℓ was favourite. Centrifuge prior to anther inoculation was improved embryoid induction at 1,000 G for 30 minutes and the frequency of embryoids obtained was about 1.5%. It was very low in order to apply breeding program. Thus, it was considered that microspore culture was more suitable than anther culture.