細胞質 遺傳子的 雄性不稔系統을 利用한 油菜 heterosis 育種에 관한 硏究 Ⅶ. 三元 交雜種 育成을 위한 莖片組織培養技術의 利用

Studies on the heterosis breeding in rape by using cytoplasmic genetic male sterile lines Ⅶ. Three-way cross hybrid development using MS line and its maintainer by stem tissue lines

This study was conducted to develope three way cross hybrid using MS maintainers derived from stem tissue culture. Seven maintainer lines of complete male sterility(100%)have been obtained and complete plants were obtained 98% by stem tissue culture. Ten differentiated plants could be obtained from one segment. The multiple shoots without roots could be divided into individual shoot and then transfered to vermiculite teralium box in order to get complete plants. The maintenance of MS lines and the production stability of three way cross hybrids could be possible by this method. Three way cross hybrids would be produced using different genetic constitution lines including MS line and its maintainer and restorer lines through tissue culture of lines with 100% male sterility maintaining ability. Commercial three way cross hybrid seed for 12,600ha could be produced from 105ha MS seed(F₁) production field through tissue culture of MS line and its maintainer MS line for seed production to be planted on 10a.