벼 葯培養에서 1次培地 植物體分化 硏究 Ⅰ. NAA와 2,4-D 混合 1次培地에 의한 植物體分化

Studies on one-step procedure in rice(oryza sativa) anther culture Ⅰ. Direct generation of rice plantlets on NAA and 2,4-D containing N6 medium

Anter culture tachnique is practically used for rice varietal development. Rice plantlets via anther culture are usually produced by a two-step procedure: callus induction and plantlet development. An one-step procedure which rice plantlets are generated directly from plated anthers without transferring the calluses to plant regeneration medium would provide the saving labor, space and time in the anter culture. Three media formulations, which cantained the mixtures of NAA(5×10⁻⁶M and 2.4-D(10⁻⁶, 10⁻⁷ and 10⁻⁸M) in the N6 medium, produced rice plantlets directly without transferring calluses to another medium. The plantlets by the one-step procedure were developed about 4 weeks after anthers were placed on the medium. The plant regeneration frequencies with the one-step procedure varied from 0% for “Taebaegbyeo” to 4.8% for “Cheonmabyeo”. In general, the media which contained 5x10⁻⁶M of NAA and 10⁻⁷~ 10⁻⁸M of 2.4-D showed better responses for the one-step procedure of rice anter culture.