Plant regeneration via somatic embryogenesis from soybean cotyledon culture

The experiment was attempted to regenerate whole plant from somatic embryo using immature cotyledon cultures of five soybean varieties under different media. Normal shape somatic embryos were formed with 53.3% under MS medium supplemented with 1.0mg 2,4-D and 0.2mg/ℓ kinetin, and 32.2% on MS medium added with 8.0mg/ℓ NAA. Root and shoot was developed from the embryos under supplement with 0.1mg IBA and 0.25 mg ℓ│2-iP│of│ B5 medium, and whole plants were regenerated under further culture on hormoneless MS medium. Ulsan variety showed a good producer for somatic embryo among the lines applied. From subcultured somatic embryo callus, embryo formation is possible although their germination and organ differentiation is not easy.