벼葯培養에서 培地 agar 濃度의 變化가 植物體 分化에 미치는 影響

Effect of high agar medium on plant regeneration in rice(oryza sativa L.) anther culture

The low frequency of plant regeneration is one of the problems in the practical appliction of anther culture technique to rice(Oryza sativa L.) breeding. In addition to the improving chemical composition, the improving physical properties of the culture medium would be necessary in terms of the controlling osmotic potential in the medium for the raising plant regeneration. Rice calli derived from four homozygous varieties and four F₁ combinations in three different callus media were evaluated for plant regeneration with four different concentrations of agar in the regeneration medium. Regardless of plant genotype and callus media, plant regeneration rate was remarkably increased with the increased agar concentration in the plant regeneration medium. The 1.6 and 1.2% agar concentrations gave the highest frequency of plant regeneration. The 1.6% agar medium was also found for the improving callus ability to sub-culture.