벼 葯培養 分化植物體의 變異性 Ⅳ. 통일형 / 자포니카형 F₁ 葯培養系統의 分類

Variability of anther-derived plants in rice(oryza sativa L.) Ⅳ. Classification of plants derived from Tongil / japonica F₁ hybrid pollen

Anthers of a cross between Tongil rice CV., Milyang 23 and Japonica rice CV., Nagdongbyeo were cultured on N6-Y1 medium, which Japonica rice was better adapted than Indica, to confirm a certain degree of screening effect of culture medium on the genetic expression of pollen plants. The salient findings obtained are summarized as follows; The majority of progeny of pollen plants, A2 lines, had horizontal leaf angle as intermediated type between parental varieties. Most of A2 lines also had the same leaf color of their parental varieties, but transgressive variation was expressed. Although most of A2 lines in grain shape distributed within parental range, transgressive variation in grain shape was also observed. The phenol color reaction of brown rice was coincide with seed integuments. The ratio of postive(+) phenol reaction to negative(-) was 1:1 in A2 population. It was considered that inheritance of the phenol color reaction was controlled by single dominant gene. The coloration of phenol solution after soaking seed integuments in 2% phenol for 5 days at 30℃ was more clearly than in brown rice. Transgressvie variation to milyang 23 observed both brown rice and seed integuments. Based on the results described above, the pollen grain of different genotype probably have the same totipotency into intact pollen plants. No significant selection effects was found during the process of anther culture. This results also provide useful information of rice breeding for Indica/Japonica hybrids through anther culture.