人蔘 休眠芽培養에 있어 生長調節物質이 休眠打破에 미치는 影響

Effects of growth regulators on dormancy breaking of dormant bud in panax ginseng C.A. meyer

This study was carried out to investigate the possibility of the shortening of generation and mass propagation by in vitro propagation of dormancy bud which is taken out from rhizome of Panax ginseng. There were investigated the effect of MS medium supplemented with GA, NAA, or IBA on dormancy breaking and rooting. Application of GA 30mg/ℓ in MS medium was effect on reducing of dormancy period and expanding of leaf. For rooting and root growth, application of IBA was more effective than that of NAA. Application of IBA 5mg/ℓ alone, or NAA 1mg/ℓ and IBA 5mg/ℓ combination resulted better effect. The rooting of the young bud was possible by treatment of IBA and NAA after dormancy breaking and leaf expansion by GA treatment. Rooting induction of old-aged ginseng was possible, however was so weaker than that of young one. Differentition of root showed vigorous growth of fine root without thickening of primary root.