煙草 半數體 植物의 葉熟度가 組織培養時 染色體數에 미치는 影響

Influence of leaf maturity to chromosome number in midrib tissue culture of haploid plant in nicotiana tabacum L.

This experiment was conducted to investigate the leaf reflectant color degree affecting the chromosome number of regenerated plants from midrib of haploid plants derived from anther culture of Nicotiana tabacum. Plants regenerated from midrib of lower leaves of haploid included diploid, haploid and tetraploid. Those regenerated from middle and upper leaves included diploid and haploid. The frequency of diploid among the regenerated plants from midrib of haploid leaves was correlated positively with lightness (r=0.82) and yellowness-blueness degree (r=0.87) of the leaf measured by color difference meter, and was correlated negatively with rednessgreenness degree (r=0.92) of the leaf.