벼 칼루스 誘起 홀몬組合과 老化程度에 따른 植物體 再分化

Plant regeneration at different callus age and callus inducing hormone combination in rice(oryza sative L.)

This experiment was carried out to know the plant regeneration frequency at the different callus age and hormone combinations for callus induction. Plant regeneration frequency of young spikelet was higher at 15 days old callus after plating of spikelet and it was decreased as increase of callus age. The decrease of regeneration ability was delayed by subculture in cv. Calrose 75. Addition of NAA to the 2,4-D was better for plant regeneration than that of use of 2,4-D alone. In cv. Calrose 75, combined effect of 3mg/ℓ 2,4-D with 2mg/ℓ NAA or 4mg/ℓ 2,4-D with 1mg/ℓ IAA were more effective for callus induction and plant regeneration, and plant regeneration freguency were 96.7% and 93.3%, respectively. In cv. Kumnampung, combined effect of 3mg/ℓ 2,4-D with 2mg/ℓ NAA or 2mg/ℓ IAA were more effective for callus induction and plant regeneration and, their regeneration frequency were 60.0% and 56.7%, respectively.