밀(triticum aestivum L.)의 葉切片으로부터 callus의 發生部位와 分裂形態에 關한 組織學的 觀察

Histological observations on origin of callus formation and its growth from leaf segments of wheat(triticum aestivum L.)

After the basal segment 0.5cm of the first leaf of wheat (Triticum aestivum L. cv. Cho-Kwang) was cultured on MS medium supplemented with 2,4-D 2mg/ℓ, whether callus was originated from epidermal cells or from procambial cells and the division shape of it were also histologically observed. The division of procambial cells, the first sign of callus formation, was started actively and hypertrophied. after that, callus was originated as cells were gradually elaborated by active cell division, and epidermal cells had no effect to callus formation. The meristem of callus was located inside the surface of callus and the division shapes of callus were irregular such as spot shape, round shape, bar shape, heart shape, triangle shape and so on, and also irregular and degenerated cellular layers at the surface of callus were observed.