生長調節物質이 人蔘 胚培養에 있어 器官分化에 미치는 影響

Effects of growth regulators on organ differentiation in embryo culture of panax ginseng C.A. meyer

This study was conducted to investigate the effect of phytohormones, IBA, GA, kinetin and BA on regeneration of plantlet, morphological characteristics of inflorescence development, growth of explant and saponin content in embryo culture of Panax ginseng. For the induction of adventitious buds and regeneration of plantlet from adventitious buds, GA was more effect than IBA, kinetin, or BA. The plantlet regeneration and inflorescence development were significantly promoted by adding 3mg/1 IBA and 3mg/1 BA together. Fresh and dry weight and saponin content of plantlet were significantly increased by adding 3mg/1 each of IBA and GA or same amount of IBA and BA. In vitro flowers were appeared at the shoot apex. The regenerated plantlets had the 2 petioles such like field grown 2 yearold ginseng.