小麥(triticum aestivum L.)葉 callus의 細胞學的 硏究 Ⅰ. 培養期間과 2,4-D 濃度에 따른 染色體數의 異常

Cytological study on callus derived from wheat(triticum aestivum L.) leaf Ⅰ. Effect of cultural period and 2,4-D concentration on aberration of chromosome number

The effects of cultural period and 2,4-D concentration on chromosome number aberration of wheat(Triticum aestivum L. cv. Cho-Kwang) leaf-derived callus were studies. Chromosome numbers were varied form 7 to even more than 42. The longer cultural period and the higher 2,4-D concentration in the culture media, the further increased cells with changed chromosome numbers. Especially, a percentage of aneuploid cells was increased. The frequency of the cells with changed chromosome numbers was prone to increase by high mitotic index of callus in concentration range from 2,4-D 0.5mg/1 to 2,4-D 2mg/1, however, in 2,4-D 5mg/1, an increase in the frequency of cells with changed chromosome numbers and decrease in both fresh weight and mitotic index were observed respectively.