벼 葯培養에서 低溫 前處理가 callus 및 植物體 分化率에 미치는 影響

Low-temperature pretreatment on callus induction and plant regeneration in rice anther culture

The increasing callus induction and plant regeneration is the first target to apply anther culture technique to rice varietal improvement. The effects of low-temperature pretreatment on the callus induction and plant regeneration were examined with different temperatures and different treatment durations in four rice varieties. Low-temperature treatment on rice anther prior to anther plating increased remarkably both callus induction and plant regeneration, especially in the callus induction. The treatment temperature was more critical than the duration. In general, the proper treatment would be 8-12℃ in temperature and 8-16 days in duration with higher temperature for Tongil type rice varieties, which were derived from indical/japonica hybridization. Highly significant correlation was found between the callus induction and plant regeneration in Tongil type rice varieties, Milyang 23 and Taebaegbyeo, while no significant correlation in Japonica varieties. The ratio of haploid and diploid plants among the anther-derived plants with lowtemperature pertreatment was varied from different rice varieties, averaging approximately 7 haploids : 3 diploids.