韓國 벼 在來種의 同位酵素 形態特性에 의한 品種區分

Classification of Korean native rice cultivars by isozyme variations

Using the method of isoelectric focusing in polyacrylamide horizontal slab gels, Korean native rice cultivars, which were collected from Gene Bank of the Rural Development Administration, were analyzed for their five isozyme patterns and classified into the enzymatic groups. Among 259 Korean native rice cultivars screened, 5 esterase(EST), 4 phosphoglucose isomerase(PGI), 4 malic enzyme(ME), 2 hexokinase(HK) and 5 β-amylase(β-AMY) isozyme phenotypes were distinguished. Total 15 enzymatic groups could be classified by combining five enzyme phenotypes. The eleventh enzymatic group 〔EST-ⅡC:ME-Ⅲ:PGI-Ⅱ:HK-Ⅱ:β-AMY-Ⅰ〕 was most frequent (111 cultivars), but only one to three cultivars were found in the 1st-6th, 9th and 13th groups. Over 96% of Korean native rice cultivars were showed the isozyme phenotypes of 〔EST-ⅡC:ME-Ⅲ:PGI-Ⅱ〕.