等電點 電氣泳動法에 의한 벼 種子內 esterase 同位酵素 遺傳子 分析

Inheritance of esterase isozymes in rice by isoelectric focusing

The inheritance of esterase isozvmes in rice was identified by controlled crosses and segregation analysis with esterase phenotypes classified. Using the method of isoelectric focusing(IEF), four esterase loci and their allozymes were identified and named as Est Ⅰ-1, Est Ⅰ-2, Est Ⅰ-3, and Est Ⅰ-4. There allozymes at each locus were found. Est Ⅰ-1 locus was controlled by two active dimeric alleles and a null allele. Two monomeric alleles and a null allele were found at both Est Ⅰ-2 and Est Ⅰ-3 locus. Two dimeric alleles and one weak allele were recognized at Est Ⅰ-4 locus. Linkage tests showed that four esterase loci were independent each other.