참깨의 種間雜種 育成에 관한 硏究 Ⅱ. S. indicum × S. radiatum間의 種間雜種 育成

Studies on interspecific hybridization in sesame Ⅱ. Development of hybrid plants from S. indicum × S. radiatum

To breed a multi-resistant midparent for several major diseases in sesame, interspecific hybridization between S. indicum (2n=26) and S. radiatum(2n=64) was attempted by the premature embryo culture method in 1988 at the green house and culture room of the Crop Experiment Station. Before the hybrid embryo culture attempted, pre-experiments were performed with selfed seed embryo in each S. indicum and S. radiatum to confirm the optimum culture time, seed part and media of which applied in premature embryo culture of the interspecific hybridization. With the application of the pre-experiment results, premature hybrid embryos were cultured on MS medium with 16-20 days old embryo. Six plants of which 2.52% of the cultured embryo were induced from the premature hybrid embryo culture of S. indicum×S. radiatum. The six plants showed intermediate of the parents in plant characteristics with 2n=45 somatic chromosomes and semi-sterility. The progenies derived from the interspecific cross is being tested of their characteristics and disease resistance in field condition.