遺傳子的 雄性不稔과 葯培養을 利用한 벼의 標識遺傳子 集積

Accumulation of marker genes by using genetic male sterility and anther culture technique in rice

Fourteen Japonica marker lines were crossed to the Japonica genetic male sterile (GMS) rice Milyang 67ms, and thirteen marker lines of Tongil type were crossed to the Tongil type GMS rice Milyang 55ms. The Fls were backcrossed to the GMS parent respectively. From the fertile BC1F1 plants, BC1F2 seeds were bulk harvested separately from Japonica group and Tongil group. These two populations of BC1F2s were grown in isolated field to facilitate composite crosses. From the male sterile plants of the BC1F2, the composite crossed seeds were harvested. The composite crossed plants were grown under green house condition in winter season, and anther culture and chromosome doubling techique were applied to get the homozygous lines for marker genes. The dihaploid plants induced from the anther culture of the composite crossed plants showed the segregation ratio of 1:1 for male sterility. Five to six marker genes could be accumulated and fixed by using GMS facilitated composite crosses and anther culture technique. The GMS facilitated composite crosses and anther culture technique was proposed as a breeding method for accumulating useful genes in rice.