벼 雄性細胞 培養에 의한 trisomicsdml 移轉과 培養細胞의 再分化에 미치는 過剩染色體의 影響

Transmission of trisomics and effect of extra chromosomes on plant regeneration through male gametic cell culture in rice

This study was carried out to investigate the transmission frequency of the extra chromosomes in rice trisomic plants through male and female gametes including selfed progeny, and to know the effect of extra chromosomes on the male gametic cell culture of rice. the extra chromosomes were transmitted to next generation at the rate of 0.0% to 9.7% through male gamete and 11.3% to 35.7% through female gamete, although the rate was 27.7% to 40.1% in selfed progeny of each trisomic type. Trisomic plant showed different culturability for callus induction, plant regeneration and green tissue appearence depending on the trisomic types in anther culture. Callus induction rates were ranged from 4.7% to 27.3% and plant regeneration rates 3.0% to 22.0%. In anther culture the highest transmission rate of extra chromosomes was 27.8% in trisomic 4(E), while the extra chromosomes of trisomic 12(A), 8(D) and 7(F) were not transmitted through anther culture in this study. The regenerated plants varied in chromosome numbers.