葯培養에 의한 들깨의 半數體植物 分化

Haploid plant production from anther culture in perilla

This experiment was conducted to select a culture medium in anther culture of perilla. Several basal medium supplemented with growth regulators were adapted in the experiment. Callus induction ability and plant regeneration efficiency were observed in each adapted medium. Callus induction rate was the highest as 86% in the MS medium supplemented with 2.Omg/ℓ 2,4-D, 1.0mg/ℓ NAA and 2.0mg/ℓ kinetin. The produced callus in the anther culture media was transfered to the plant regeneration culture media of which developed plantlets. On MS medium supplemented with 0.1mg/ℓ NAA and 0.5mg/ℓ BA induced the highest frequency of shoot as 66.9% compared to any other medium tested. The multiple shoots removed roots could be regenerated into individual shoot on MS medium with 0.1mg/ℓ NAA and 0.5mg/ℓ BA and then transfered to teralium box in order to get complete plant. Complete plants were obtained 100% after translanting in soil bed type of vermiculite 1:sand 1:compost 1. Regenerated plants from anther culture were confirmed as haploid with n=20 haploidal somatic chromosome number in the root tip chromosome observation.