Para-fluorophenylalanine(PFP) 처리에 의한 작물의 염색체 변이 Ⅰ. 배지, PFP 및 hormone이 메밀 및 담재 callus 유기 및 생장에 미치는 영향

Chromosomal variation in crops by para-fluorophenylalanine(PFP) Ⅰ. Effects of culture media, PFP and hormones on the induction and growth of callus of buckwheat and tobacco

The callus induction ratio and the callus growth ratio of buckwheat and tobacco were investigated using the different kinds of media and hormones and the different concentrations of PFP and hormones to determine the optimum conditions of callus culture. The highest ratios of callus induction in buckwheat and tobacco were respectively observed in the medium containing 1mg/1 2,4-D in the dark (68.4%) and in the B₅ medium containing 2mg/1 2,4-D in the light (82.5%). In tobacco, callus induction ratio was decreased as PFP concentrations increased in the mixture of 2,4-D and PFP as well as in the addition of PFP alone. None of callus was formed over 10mg/1 PFP in the mixture of 2,4-D and PFP while callus induction of 5% was observed even in 80mg/1 PFP in the addition of PFP alone. Dicamba in the media MS and NN affected better callus growth of buckwheat than 2,4-D and picloram did. Fresh whight of tobacco callus increased markedly in the MS medium compared to the media B₅ and NN. Fresh weight of buckwheat callus was more increased in the mixture of 2,4-D and PFP rather than in the addition of PFP alone while that of tobacco callus was higher in the addition of PFP alone.