眞珠조ㆍ옥수수ㆍ수수類 飼料作物의 靑酸含量의 變異

Genetic variations of hydrocyanic acid potential in pearl millet, maize and sorghum/sudangrass hybrids

Spectrophotometric assay method was used to determine the hydrocyanic acid content(HCN) in pearl millet, maize, sorghum and sorghum/sudangrass hybrid. Uniform samples for the comparison of widely divergent genotypes of the crops examined were obtained using the third leaf of the young green seedlings grown on the bed of green house. HCN was extracted and hydrolysed by autoclaving the young leaf tissue in water. The HCN content in the aqueous extract was determined by spectrophotometric assay in alkaline solution at 330nm. The mean hydrocyanic acid potential(HCN-p) values for the crop’s genotypes assayed for HCN content by this technique were very low in pearl millet and maize as compared with sorghum and sorghum/sudangrass hybrid. The mean HCN-p values of the fresh leaves were 0.06216ppm in pearl millet, 0.06816ppm in maize, 0.16776ppm in sorghum, and 0.11740ppm in sorghum/sudangrass hybrid.