種子蛋白質의 SDS-PAGE法에 의한 보리(hordeum vulgare. L)의 品種分類

Classification of barley cultivars through SDS-polyacrylamide gel electrophoresis of seed protein

This study was carried out to identify the barley cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of seed protein. The 23 Korean barley cultivars, 9 covered, 7 naked and 7 malting barely and four foreign cultivars, Carlsberg Ⅱ, Proctor, Jupiter, Vada were used as materials. There was no variation of hordein band patterns among spring, winter and greenhouse cultivation of Doosan # 8 and Doosan # 29. B hordeins were grouped into 11 and C hordeins into 9 and totally grouped into 14 different types by SDS-PAGE of hordeins from 23 Korean barley cultivars and 4 foreign cultivars. The relationships among 27 barley cultivars based on SDS-PAGE band patterns of hordeins were mostly corresponded with the genealogical diagrams of Korean cultivars.