油菜의 小胞子培養에 의한 胚狀體發生과 植物體分化

Embryogenesis and plant regeneration in rapeseed microspore culture

Fifteen genotypes of rapeseed were used in the study of embryogenesis and temeprature treatment to the embryo induced from microspore culture. Embryo yields, frequency of normal embryo production and plant regeneration rate were greatly different between genotypes and between plants within a genotype. Embryo yield ranged from 6 to 302 embryos per bud, normal embryos ranged from 7 to 84.1% of total embryos produced, and plant regeneration from embryos showed from 0 to 35.4%, of embryos subcultured. Plant regeneration from embryo age of 28 days was most efficient by cold temperature treatment at 4℃ and exposure times for 9 days.