油菜小胞子 發育時期가 胚狀體發生에 미치는 影響

Effects of developomental stages of microspores on embryogenesis in rapeseed

To obtain the basic information for rapeseed breeding, embryogenesis by microspore-developmental stages and the relationship between anther length and microspore-developmental stages were investigated. The embryogenesis by bud sizes were most efficient from 3.5 to 4.5mm for Topas and from 2.8 to 3.3 mm for MSB 69-11, and the anther lengths were from 2.0 to 2.9mm. The best response for the tested varieties was from buds with a petal/anther ratio of 2/3 and 3/4. The late uninucleate stage has been identified as optimal for embryo production in B. napus. At the late uninucleate stage the exine was rigid, dark yellow in color and the nucleus was displaced to the periphery of the cell.