매립지 침출수로 오염된 지하수의 미생물 군집 구조 특성

Characterization of microbial community in groundwater contaminated with landfill leachate

The microbial community properties of groundwater samples contaminated with landfill leachate were examined by two methods: (1) Ecoplate including 31 sole carbon sources and (2) DGGE method using PCR (polymerase chain reaction) products of 16S rDNA. The samples were contaminated groundwater (KB01~03, KB05~09, KSG07, 11), uncontaminated ground water (KB10), leachate (LA), and treated leachate (NMW01). Average well color development (AWCD) analysis demonstrated that the potential activity on 31 substrates was highest in KB10, which was farthest (except KSG11) from the landfill and lowest in KB02 and KSG07, which were closest to the landfill. Principal component analysis (PCA) on similarity between samples did not show a specific grouping but NMW01 was much different from the others, coinciding with lack of nutrients probably due to treatment. Shannon index showed that the microbial diversities were similar among the samples, except KSG07 assuming high contamination. In the comparison of DGGE fingerprint patterns, the similarity was highest between KB05 and KB08 (53.8%), probably due to close site location and lowest between NMW01 and KB03 (10.0%), assumingly due to different contamination levels.