빙핵활성단백질의 N-terminal부분을 이용한 녹색형광단백질의 Zymomonasmobilis세포 표면 발현

Display of green fluorescentprotein (GFP)on the cell surface of Zymomonas mobilis using N-terminal domain of ice nucleation protein

Green fluorescent protein (GFPuv) was displayed on the surface of ethanol-producing bacteria Zymomonas mobilis using N-terminaldomain of ice nucleation protein (INP) as an anchoring motif. To evaluate the ice nucleation protein as plausible anchor motif in Z. mobilis, GFPuv gene was subcloned into Zymomonas expression vector yielding pBBR1MCS-3/pPDC/INPN/GFPuv plasmid., INP-GFPuv fusion protein was expressed in Z.mobilis and its fluorescence was verified by confocal microscopy. The successful display of GFPuv on Zymomonas mobili ssuggest that INP anchor motif could be used for future fusion partner in Z. mobili sstrain improvement.